2b lanciotti

Information about 2b lanciotti

Published on October 24, 2007

Author: Esteban

Source: authorstream.com

Content

Serological and Molecular Assays for West Nile Virus:  Serological and Molecular Assays for West Nile Virus Current Techniques & Improvements Recommended Tests for WN Virus:  Recommended Tests for WN Virus Slide3:  Serological Assays for West Nile Virus human serum/csf equine serum avian (chicken) IgM ELISA IgG ELISA POS NEG PRNT with: SLE, WN, DEN STOP Slide4:  WN Serological Data IgM ELISA P/N for WN > 2X IgM P/N to SLE. PRNT titer to WN 4-fold higher than to SLE or DEN 4-fold rise in PRNT titer to WN from acute to conv. specimen Typical Human WN Case 83% of 2001 WN cases met criteria Slide5:  WN Serological Data from 2001 IgM ELISA P/N for WN < IgM P/N to SLE. PRNT titer to WN 4-fold higher than to SLE or DEN Slide6:  WN Serological Data from 2001 IgM ELISA P/N for WN > 2X IgM P/N to SLE. 4-fold rise in PRNT titer to WN from acute to conv. Specimen Flavivirus IgG in acute serum WN & SLE PRNT titer approximately equal. Slide7:  Virus/Antigen Detection Assays for West Nile Virus mosquito pools avian/equine/other tissues human csf 1. NASBA & TaqMan (0.1 pfu) 2. Virus isolation (1 pfu) Antigen capture ELISA, RT-PCR & VecTest (10 pfu) Slide8:  Antigen-capture ELISA VecTest (WN & SLE) 18/30 (60%) TaqMan positive pools positive. Oral Swabs of avian carcasses Crows & blue jays 100% agreement with assay of brain tissues New Developments in Virus/Antigen Detection Assays for West Nile Virus 15 min Instruments for TaqMan Assays:  Instruments Evaluated at CDC PE-ABI 7700 – in use BioRad iCycler – in use PE-ABI 7000 MJ Research DNA Engine OPTICAN Available/Not Evaluated at CDC Cepheid SmartCycler Stratagene Mx4000 Corbett Research RotorGene Instruments for TaqMan Assays TaqMan Multiplex Assays for Arboviruses:  TaqMan Multiplex Assays for Arboviruses WN, SLE, and LAC detected in a single reaction. Up to 4 probes could be utilized (i.e. EEE). Instrument capable of multiplexing. WN-FAM probe SLE-CY5 probe LAC-HEX probe Diagnostic & Reference Section TaqMan & NASBA Assays :  Diagnostic & Reference Section TaqMan & NASBA Assays

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