AAA website video Version Final

Information about AAA website video Version Final

Published on December 8, 2009

Author: cwigmore

Source: authorstream.com

Content

Slide 1: UPLC® Technologyfor Amino Acid Analysis Challenges for Amino Acid Analysis : Challenges for Amino Acid Analysis Difficult analytical problem Separation Wide range of properties Slight differences between pairs Wide range of matrices Detection No chromophore Wide concentration range Analytical method requirements Unequivocal identification, accuracy Precision and linearity Sensitivity and speed Rugged and robust method UPLC® Application Solution for Amino Acid Analysis : UPLC® Application Solution for Amino Acid Analysis Turn-key UPLC® Amino Acid Analysis application Optimized for the ACQUITY UPLC System Dedicated QC-tested column and reagents Application-specific Performance Qualification Same result day-to-day, instrument-to-instrument, lab-to-lab, around the world Target Applications Protein and peptide ID and quantitation Monitoring cell culture media Nutritional content of food and feed Criteria for Optimized Solution : Criteria for Optimized Solution Based new method on ACQUITY UPLC® System and TUV detector Highest resolution for unambiguous identification Good sensitivity and 3 orders of magnitude linear dynamic range for quantitation Well-controlled column chemistry - QC tested with application Mobile Phase Tested in QC Separation selectivity tolerates pH and ionic strength changes Criteria for Optimized Solution : Criteria for Optimized Solution Based new method on ACQUITY UPLC® System and TUV detector Highest resolution for unambiguous identification Good sensitivity and 3 orders of magnitude linear dynamic range for quantitation Well-controlled column chemistry - QC tested with application Mobile Phase Tested in QC Separation selectivity tolerates pH and ionic strength changes Based new method on Empower™ Software Fully-compliance ready Method templates Custom calculations Criteria for Optimized Solution : Criteria for Optimized Solution ACQUITY UPLC® System and TUV etector Highest resolution for unambiguous identification Good sensitivity and 3 orders of magnitude linear dynamic range for quantitation Well-controlled column chemistry - QC tested with application Mobile Phase Tested in QC Separation selectivity tolerates pH and ionic strength changes Empower™ Software Fully-compliance ready Method templates Custom calculations AccQ-Tag™ Derivatization Chemistry of AQC Derivatization : Chemistry of AQC Derivatization Established and well understood chemistry Reacts readily with both primary and secondary amines Forms stable derivatives No special hydrolysis or sample preparation Requires no vacuum drying, sample prep or extraction Amendable to automation UPLC® Amino Acid AnalysisApplication Solution : UPLC® Amino Acid AnalysisApplication Solution UPLC® Amino Acid AnalysisApplication Solution : UPLC® Amino Acid AnalysisApplication Solution Reproducibility:50 pmol on Column : Reproducibility:50 pmol on Column Amino Acid AnalysisApplication Types : Amino Acid AnalysisApplication Types Amino Acid Analysis Protein Hydrolysates Cell Culture Food Analysis Physiological Clinical

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