DNA From Stool

Information about DNA From Stool

Published on July 15, 2014

Author: mobio

Source: authorstream.com


DNA From Stool: DNA From Stool PowerPlant® Pro DNA Isolation Kit : PowerPlant ® Pro DNA Isolation Kit For fast and easy isolation of inhibitor-free genomic DNA from even the toughest plant and seed samples, including those high in polyphenols and polysaccharides. ON SALE NOW! (Sale price is valid through 3/31/14 for US direct web orders only) RTS DNase™ Kit: RTS DNase™ Kit RTS (Room Temperature Stable) DNase is a highly purified DNase I enzyme formulated in a unique stabilization solution that provides long term stability at room temperature. The RTS DNase™ Kit is used for the removal of genomic DNA contamination in RNA preparations. RTS DNase will remove up to 30 µg of DNA in 20 minutes using 10 units (1 µl) of enzyme. The enzyme is stable for up to 6 months at room temperature with no loss of activity and for 2 years at 4°C without loss of activity. PowerPoint Presentation: DNA-Free Certification This test certifies any of a wide variety of products to be free of certain types of DNA and common contaminants and inhibitors that interfere with the PCR process . Test samples are extracted and a portion of extract is added to a multiplex PCR reaction containing primers specific for human and mouse genomic DNA. These tubes receive no template DNA so any amplification in these tubes will indicate the presence of contaminating DNA. A negative control reaction is done with DNA-Free water as a reference. In another set of reactions that test for PCR inhibition, DNA is added to tubes containing the multiplex PCR reaction and product extract. A positive control reaction is performed with DNA-Free water as a reference. PowerPoint Presentation: RNase -Free Certification A wide variety of products are certified to be free of RNase contamination and ready for direct use in all RNA work using this test . All testing apparatus are treated with Diethyl Pyrocarbonate (DEPC) to inhibit possible RNase contamination of the experiment from outside sources. Test samples of the product are extracted and a portion of extract is incubated with an RNA standard (6 kb RNA) in a buffered solution with both sodium and magnesium ions. Negative and Positive controls are run with the test sample extract to validate the experimental procedure and technique. An unexposed RNA standard is included to represent the negative control. An RNA standard exposed to RNase is also included to represent the positive control. PowerPoint Presentation: ATP-Free Certification This test is used to certify a variety of products to be free of contaminating ATP molecules which can interfere with several types of enzymatic reactions and also further indicate the presence of any biological activity . A luciferase-based assay is used to detect the level of ATP in a sample of product. Luciferase is the enzyme found in fireflies that causes them to emit light. The luciferase enzyme catalyzes an ATP dependent oxidation of the luciferin substrate (shown in the reaction below ). ATP + D Luciferin + O2 --> Luciferase --> AMP + oxyluciferin + CO + PPi + light PowerPoint Presentation: For more information Please visit http://www.mobio.com

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