Evaluatio of hypoglycemic activity of Aegle marmelos fruit

Information about Evaluatio of hypoglycemic activity of Aegle marmelos fruit

Published on April 17, 2011

Author: DeepikaGupta

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Evaluation of Hypoglycemic Activity of Aegle marmelos FruitGupta D, Kaushik R, Verma R, Dr. Mohd. Shuaib , John P. P.Ram Eesh Institute of Vocational & Technical Education, Department of Pharmacy, Greater Noida : Evaluation of Hypoglycemic Activity of Aegle marmelos FruitGupta D, Kaushik R, Verma R, Dr. Mohd. Shuaib , John P. P.Ram Eesh Institute of Vocational & Technical Education, Department of Pharmacy, Greater Noida Aegle marmelos Corr. (Rutaceae), commonly known as Bael, Bengal Quince is a plant indigenous to Uttar Pradesh, Madhya Pradesh, Bihar and other parts of India. Traditionally the fruit of this plant is used in the treatment of diabetes, diarrhoea and dysentery .The objective of the study is to assess the antidiabetic potential of unriped ethanolic (50%) extract of bael fruit. The fruit is collected authenticated, shade dried, pulverized into coarse powder and is subjected to soxhlet extraction. The liquid extract is concentrated under vaccum and the resulting semisolid mass was preserved in well closed container. For evaluation of antidiabetic activity , albino rats weighing between 150- 200 g are divided into 6 groups , the animals of remaining groups are fasted for 24 hr. and diabetes is induced by alloxan monohydrate ( 150 mg / kg , i.p.) After 48 hr. of diabetes induction the treatment is given. Group 1 serves as control which receives normal saline solution through oral route. Group 2 serves as diabetic control. Group 3 receives glibenclamide (10 mg / kg) as reference standard. Group 4, 5 and 6 animals will be given aqueous extract orally at a dose of 200, 400 and 600 mg / kg respectively. Blood samples are withdrawn at 0 hr, 1 hr, 2 hr, 3 hr, 4 hr and 24 hrs. After treatment from retro orbital plexus, are analyzed for the blood glucose level using glucometer. The results are expected to show more significant activity for the fruit extract in acute and prolonged treatment and should be comparable with glibenclamide. Aegle marmelos Corr (Rutaceae) is highly reputed ayurvedic medicinal tree commonly known as the bale fruit tree, is medium sized tress growing throughout the deciduas forest of India of altitude 1200 meter. It is found whole over India, from sub-Himalayan forest, Bengal, central and south India. All the parts of the tree viz, root, leaf, trunk and fruit are used in traditional system of medicine. Fruits are globose, grey, or yellowish, upto 20 cm in diameter, with woody rind. Seeds numerous, oblong, compressed, embedded in sacs covered with thick, orange coloured sweet pulp root bark is 3 to 5 cm thick covered, with creamy yellowish surface. The surface is rough, irregular and shallow with ridges along the line of development of lenticels cream colored flaring edges. It has a firm leathery texture, a sweet taste and fracture is fibrous. T.S. of Bael Fruit Dried Form The pulp shows the group of orange coloured parenchymatous cells filled with yellowish orange content which dissolves in concentrated H2SO4 and gives bright magenta colour . Fragments of vessels with spiral , scalariform and reticulate secondary walls thickenings are also seen. The chief constituents responsible for antidiabetic activity are marmelosin , maemesinin , aurapten and psoralen. There is as much as 9% tannin in the pulp of wild fruits, less in the cultivated types. The rind contains up to 20%. It contains many vitamins like vitamin C, vitamin A, thiamine, riboflavin, niacin, and minerals like calcium, and phosphorus The fruit has found to show potent antidiabetic activity due to presence of coumarins in the fruit which potentiates insulin secretion. Fresh half-ripe Bael fruit is mildly astringent and is used in India for dysentery and diarrhoea; the pulp may be eaten or the decoction administered. The fruit dried extract have also reported to show antihyperlipidemic, antioxidant , antibacterial , antifungal and antiviral activities. Bael Fruit Parenchymatous cells (b) Fragments of spiral , scalariform & reticulate secondary walls thickenings Psoralen Marmelosin Marmin Aurapten Pharmacological activities STANDARDIZATION PARAMETERS Phytochemical Screening EXTRACTION PROCEDURE The fruit is collected authenticated, shade dried, pulverized into coarse powder and is subjected to soxhlet extraction with 50% ethanol. The liquid extract is concentrated under vaccum and the resulting semisolid mass was preserved in well closed container. BIOLOGICAL SCREENING CONCLUSION The results are expected to show more significant activity for the fruit extract in acute and prolonged treatment and should be comparable with glibenclamide. REFERENCES ABSTRACT Dr. Mukherjee Pulok K (2002);”Quality Control of Herbal Drugs”; Business Horizon Publishers; Edition first ; pp 693. Rathi Anshu*,Rai Vartika, Evaluation of Ayurvedic compound formulation III - Laghugangadhar Churna Indian Journal of Traditional Knowledge, Vol 9(3) , july 2010, pp. 576-580. Maity Pallab, Hansda Dhananjay, Biological activities of crude extracts and chemical constituents of Bael, Aegle marmelos(L) Corr. , Indian Journal of Experimental Biology, Vol. 47, november 2009, pp. 849-861. Yaheya Mohammad, Ismail Mohammad, Clinical Evaluation of Antidiabetic Activity of Trigonella seeds and Aegle marmelos leaves, World Applied Science Journal 7(10) : 1231- 1234, 2009, ISSN 1818- 4952. INTRODUCTION MICROSCOPY MORPHOLOGY PHYTOCONSTITUENTS

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