Femi April20

Information about Femi April20

Published on September 24, 2007

Author: GenX

Source: authorstream.com

Content

Slide1:  WRKY70 Mediates Cross-talk Between Plant Defense Pathways Citation: Li Jing, G. Brader and T. Palva 2004. The WRKY70 transcription factor: A node of convergence for jasmonate-mediated and salicylate- mediated signals in plant defense. The Plant Cell 16: 319-331 Olufemi Alabi Slide2:  Slide3:  Slide4:  Slide5:  Slide6:  Slide7:  Slide8:  Slide9:  Slide10:  Slide11:  Slide12:  Objective 1 Identification of the transcription factor involved in the cross talk between SA- and JA/ET-dependent signal transduction pathways in the Arabidopsis/E. c. carotovora system Slide13:  www.clontech.com Slide14:  Slide15:  Characterization of the expression of the expression of the identified TF in response to plant hormones and pathogen elicitors Objective 2 WHAT INDUCES THE EXPRESSION OF WRKY70? thaliana wild-type plants treated with pathogen elicitors RNA blot analysis to characterize expression of WRKY70 in response to elicitors Gas chromatography used in assessing accumulation of SA and JA in CF-treated leaves Slide16:  Slide17:  Characterization of the expression of the expression of the identified TF in response to plant hormones and pathogen elicitors Objective 2 (B) WHAT PLANT DEFENSE SIGNAL IS INVOLVED IN THE EXPRESSION OF WRKY70? NahG plants; plants mutated for npr1-1, ein2-1 and coi1-1 Basal expression of WRKY70 assessed in the plants using RNA blot analysis WRKY70 expression assessed after CF and SA treatment Slide18:  Slide19:  Characterization of the expression of the identified TF in response to plant hormones and pathogen elicitors Objective 2 (C) ANY FUNCTION FOR WRKY70 DURING PLANT DEFENSE? Agrobacterium-mediated transformation used to generate S55-WRKY70-overexpressing and A18-WRKY70 suppressing plants Transformants challenged with CF Visual observation of inoculated plants RNA blot analysis Slide20:  Slide21:  Slide22:  Characterization of the expression of the expression of the identified TF in response to plant hormones and pathogen elicitors Objective 2 (D) DOES WRKY70 AFFECT ENDOGENOUS LEVELS OF JA, ET AND SA? S55 WRKY70-overexpressing and A18-WRKY70 suppressing plants Extraction of SA, ET and JA homologues Gas-chromatography Slide23:  Slide24:  Identification of target genes controlled by WRKY70 Objective 3 IS WRKY70 DIRECTLY INVOLVED IN THE CONTROL OF THE EXPRESSION OF PR GENES? S55 WRKY70-overexpressing and A18-WRKY70 suppressing plants RNA blot analysis Slide25:  Slide26:  Study of the gene regulation mechanism of WRKY70 in transgenic plants Objective 4 (A) WHERE IS WRKY70 POSITIONED IN AN SA-DEPENDENT SIGNAL PATHWAY? S55 WRKY70-overexpressing, NahG, and npr1-1 plants Epistasis analysis RNA blot analysis Slide27:  Slide28:  SA WRKY70 WRKY70 Slide29:  Study of the gene regulation mechanism of WRKY70 in transgenic plants Objective 4 (B) EFFECT OF WRKY70 POSITIONED ON JA-INDUCIBLE GENES? S55 WRKY70-overexpressing and A18-WRKY70 suppressing plants; npr1-1 mutant plants RNA blot analysis using PDF1.2 marker Slide30:  4A Slide31:  Study of the gene regulation mechanism of WRKY70 in transgenic plants Objective 4 (C) LEARNING MORE ABOUT THE FUNCTION OF WRKY70 IN A. thaliana DEFENSE RESPONSES S55 WRKY70-overexpressing and A18-WRKY70 suppressing plantsRNA Microarray Slide32:  Slide33:  Slide34:  Model Showing WRKY70-mediated Cross-talk Between SA- And JA-dependent Defense Signaling Slide35: 

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