Nucleic Acids

Information about Nucleic Acids

Published on February 24, 2014

Author: drmustansar

Source: authorstream.com

Content

PowerPoint Presentation: Nucleic Acids PowerPoint Presentation: Information encoded in a DNA molecule is transcribed via synthesis of an RNA molecule The sequence of the RNA molecule is "read" and is translated into the sequence of amino acids in a protein. Nucleic Acids Are Essential For Information Transfer in Cells Central Dogma of Biology: Central Dogma of Biology Replication DNA replication yields two DNA molecules identical to the original one, ensuring transmission of genetic information to daughter cells with exceptional fidelity. Transcription The sequence of bases in DNA is recorded as a sequence of complementary bases in a single-stranded mRNA molecule. Central Dogma of Biology: Central Dogma of Biology Translation Three-base codons on the mRNA corresponding to specific amino acids direct the sequence of building a protein. These codons are recognized by tRNAs (transfer RNAs) carrying the appropriate amino acids. Ribosomes are the “machinery” for protein synthesis. Nucleic Acids: Nucleic Acids First discovered in 1869 by Miescher. Found as a precipitate that formed when extracts from nuclei were treated with acid. Compound contained C, N, O, and high amount of P. Was an acid compound found in nuclei therefore named nucleic acid Nucleic Acids: Nucleic Acids 1944 Oswald, Avery, MacLeod and McCarty demonstrated that DNA is the molecule that carrier genetic information. 1953 Watson and Crick proposed the double helix model for the structure of DNA Nucleic Acids: Nucleic Acids Nucleic acids are long polymers of nucleotides. Nucleotides contain a 5 carbon sugar, a weakly basic nitrogenous compound (base), one or more phosphate groups. Nucleosides are similar to nucleotides but have no phosphate groups. PowerPoint Presentation: Pentoses of Nucleotides D-ribose (in RNA) 2-deoxy-D-ribose (in DNA) The difference - 2'-OH vs 2'-H This difference affects secondary structure and stability Nitrogenous Bases: Nitrogenous Bases Bases are attached by b-N-glycosidic linkages to 1 carbon of pentose sugar – (Nucleoside): Bases are attached by b-N-glycosidic linkages to 1 carbon of pentose sugar – (Nucleoside) Nucleosides: Nucleosides Base is linked via a b-N-glycosidic bond The carbon of the glycosidic bond is anomeric Named by adding -idine to the root name of a pyrimidine or -osine to the root name of a purine Conformation can be syn or anti Sugars make nucleosides more water-soluble than free bases PowerPoint Presentation: Anti- conformation predominates in nucleic acid polymers Nucleotides: Nucleotides Phosphate ester of nucleosides The plane of the base is oriented perpendicular to the plane of the pentose group: The plane of the base is oriented perpendicular to the plane of the pentose group PowerPoint Presentation: Other Functions of Nucleotides Nucleoside 5'-triphosphates are carriers of energy Bases serve as recognition units Cyclic nucleotides are signal molecules and regulators of cellular metabolism and reproduction PowerPoint Presentation: ATP is central to energy metabolism GTP drives protein synthesis CTP drives lipid synthesis UTP drives carbohydrate metabolism PowerPoint Presentation: Nucleotide monomers are joined by 3’-5’ phosphodiester linkages to form nucleic acid (polynucleotide) polymers Nucleic Acids: Nucleic Acids Nucleic acid backbone takes on extended conformation. Nucleotide residues are all oriented in the same direction (5’ to 3’) giving the polymer directionality. The sequence of DNA molecules is always read in the 5’ to 3’ direction Bases from two adjacent DNA strands can hydrogen bond: Bases from two adjacent DNA strands can hydrogen bond Guanine pairs with cytosine Adenine pairs with thymine Base pairing evident in DNA compositions: Base pairing evident in DNA compositions Base compositions experimentally determined for a variety of organisms PowerPoint Presentation: H-bonding of adjacent antiparallel DNA strands form double helix structure Properties of DNA Double Helix: Properties of DNA Double Helix Distance between the 2 sugar-phosphate backbones is always the same, give DNA molecule a regular shape. Plane of bases are oriented perpendicular to backbone Hydrophillic sugar phosphate backbone winds around outside of helix PowerPoint Presentation: Noncovalent interactions between upper and lower surfaces of base-pairs (stacking) forms a closely packed hydrophobic interior. Hydrophobic environment makes H-bonding between bases stronger (no competition with water) Cause the sugar-phosphate backbone to twist. View down the Double Helix: View down the Double Helix Sugar-phosphate backbone Hydrophobic Interior with base pair stacking Structure of DNA Double Helix: Structure of DNA Double Helix Right handed helix Rise = 0.33 nm/nucleotide Pitch = 3.4 nm / turn 10.4 nucleotides per turn Two groves – major and minor PowerPoint Presentation: Within groves, functional groups on the edge of base pairs exposed to exterior involved in interaction with proteins. Factors stabilizing DNA double Helix: Factors stabilizing DNA double Helix Hydrophobic interactions – burying hydrophobic purine and pyrimidine rings in interior Stacking interactions – van der Waals interactions between stacked bases. Hydrogen Bonding – H-bonding between bases PowerPoint Presentation: Charge-Charge Interactions – Electrostatic repulsions of negatively charged phosphate groups are minimized by interaction with cations (e.g. Mg 2+ )

Related presentations


Other presentations created by drmustansar

glycogen metabolism
27. 02. 2014
0 views

glycogen metabolism

GLYCOGEN STORAGE DISEASES
27. 02. 2014
0 views

GLYCOGEN STORAGE DISEASES

STUCTURE OF NUCLEIC ACID
24. 02. 2014
0 views

STUCTURE OF NUCLEIC ACID

protein
24. 02. 2014
0 views

protein

LIPID MOBILIZATION
24. 02. 2014
0 views

LIPID MOBILIZATION

Digestion and Absorption
24. 02. 2014
0 views

Digestion and Absorption

4D STRUCTURE OF PROTEINS
22. 02. 2014
0 views

4D STRUCTURE OF PROTEINS

carbohydrate chemistry
22. 02. 2014
0 views

carbohydrate chemistry

anemia   and   vitamins
10. 03. 2014
0 views

anemia and vitamins

Success
11. 03. 2014
0 views

Success

Tulips for all
12. 03. 2014
0 views

Tulips for all

Colours only God Can Create
12. 03. 2014
0 views

Colours only God Can Create

Amino Acid Catabolism
22. 02. 2014
0 views

Amino Acid Catabolism

SECRETS OF LIFE
14. 03. 2014
0 views

SECRETS OF LIFE

keys to living life
20. 03. 2014
0 views

keys to living life

cofactors
12. 02. 2014
0 views

cofactors

HEPTOLOGY
19. 03. 2014
0 views

HEPTOLOGY

SEASON OF TULIPS
12. 03. 2014
0 views

SEASON OF TULIPS

springs
31. 03. 2014
0 views

springs

vaccine production techniques
01. 04. 2014
0 views

vaccine production techniques

Beautiful Rabbits
21. 04. 2014
0 views

Beautiful Rabbits

jaundice  biochemical profile-
23. 05. 2014
0 views

jaundice biochemical profile-

PORPHYRIA
23. 05. 2014
0 views

PORPHYRIA

heme synthesis
23. 05. 2014
0 views

heme synthesis

let go of  these  habits
27. 05. 2014
0 views

let go of these habits

12 secrets for successful life
05. 06. 2014
0 views

12 secrets for successful life

Quotes that inspire Greatness
10. 06. 2014
0 views

Quotes that inspire Greatness

nature   photographs
10. 07. 2014
0 views

nature photographs

facts about women
13. 07. 2014
0 views

facts about women

Carbohydrates summary
13. 10. 2014
0 views

Carbohydrates summary

chemistry of aminoacids
14. 10. 2014
0 views

chemistry of aminoacids

monosaccharides-
14. 10. 2014
0 views

monosaccharides-

ketosis
14. 10. 2014
0 views

ketosis

fatty acid oxidation
14. 10. 2014
0 views

fatty acid oxidation

Rna
15. 10. 2014
0 views

Rna

m phil thesis
27. 10. 2014
0 views

m phil thesis

thesis mphil
31. 10. 2014
0 views

thesis mphil

CV MUSTANSAR
29. 10. 2014
0 views

CV MUSTANSAR

DR MUHAMMAD MUSTANSAR
05. 11. 2014
0 views

DR MUHAMMAD MUSTANSAR

CHOLESTEROL METABOLISM
18. 11. 2014
0 views

CHOLESTEROL METABOLISM

LIPO PROTEINS
20. 11. 2014
0 views

LIPO PROTEINS

LACTATION
21. 03. 2015
0 views

LACTATION

Dietary Plan for Diabetes
29. 03. 2015
0 views

Dietary Plan for Diabetes

tissue processing
17. 03. 2015
0 views

tissue processing

dr mustansar lahore
03. 07. 2015
0 views

dr mustansar lahore

SOCIAL MEDIA USEAGE
29. 03. 2016
0 views

SOCIAL MEDIA USEAGE